It was confirmed that the Argentine will miss Sunday’s clash with Eibar.
The Camp Nou side will finish their campaign with a trip to Eibar.
However, they have little to play for after being ruled out of the title race with a 2-1 loss to Celta Vigo last week.
But they will line up without their talisman as Messi has been given permission by Ronald Koeman to sit out the weekend’s game.
Messi out of the game
“The Argentine Leo Messi has not trained on Friday with the coach’s permission,” the club said through a statement.
“He will not be in the squad for the game against Eibar on Saturday.”
“The striker can enjoy a rest before next month’s Copa America after a season.”
“He is one of the players in the squad who has played most.”
Meanwhile, Messi’s contract at the Catalan club expires at the end of the season.
Messi announced last year that he wanted to leave on a free transfer.
However, Barca remain hopeful of convincing him to stay.
Team-mate Jordi Alba has urged him to stay at the club.
Manchester City have been touted as one of the leading candidates to sign him due to their financial strength.
On top of this is the presence of former Barcelona coach Pep Guardiola.
But Paris Saint-Germain are also said to be in the race as they hope to reunite him with his old team-mate Neymar.